Detailed information of TA system TA071565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3033520..3034122
• Replicon: chromosome
• Accession: NZ_CP019029
• Organism: Escherichia coli strain Ecol_881

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BE930_RS18045
• Protein ID: WP_000897305.1
• Coordinates: 3033811..3034122 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE930_RS18040
• Protein ID: WP_000356397.1
• Coordinates: 3033520..3033810 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE930_RS18015	3030022..3030924	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BE930_RS18020	3030921..3031556	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BE930_RS18025	3031553..3032482	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BE930_RS18030	3032697..3032915	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
BE930_RS18040	3033520..3033810	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BE930_RS18045	3033811..3034122	-	312	WP_000897305.1	hypothetical protein	Toxin
BE930_RS18050	3034351..3035259	+	909	WP_001318161.1	alpha/beta hydrolase	-
BE930_RS18055	3035323..3036264	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE930_RS18060	3036309..3036746	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BE930_RS18065	3036743..3037615	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BE930_RS18070	3037609..3038208	-	600	WP_001314324.1	glucose-1-phosphatase	-
BE930_RS18075	3038307..3039092	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T71565 WP_000897305.1 NZ_CP019029:c3034122-3033811 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71565 NZ_CP019029:c3034122-3033811 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71565 WP_000356397.1 NZ_CP019029:c3033810-3033520 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71565 NZ_CP019029:c3033810-3033520 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7