Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2047480..2048174 Replicon chromosome
Accession NZ_CP019029
Organism Escherichia coli strain Ecol_881

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag BE930_RS12950 Protein ID WP_001263491.1
Coordinates 2047480..2047878 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag BE930_RS12955 Protein ID WP_000554755.1
Coordinates 2047881..2048174 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE930_RS12920 2042742..2044199 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
BE930_RS12925 2044208..2044489 + 282 WP_022645226.1 hypothetical protein -
BE930_RS12930 2044506..2045015 - 510 WP_001361775.1 hydrolase -
BE930_RS12935 2045077..2045691 - 615 WP_022645225.1 peptide chain release factor H -
BE930_RS12940 2045688..2046827 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
BE930_RS12945 2047018..2047470 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
BE930_RS12950 2047480..2047878 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BE930_RS12955 2047881..2048174 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BE930_RS12960 2048226..2049281 - 1056 WP_022645222.1 DNA polymerase IV -
BE930_RS12965 2049352..2050137 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
BE930_RS12970 2050109..2051821 + 1713 Protein_1985 flagellar biosynthesis protein FlhA -
BE930_RS12975 2051919..2052692 - 774 WP_022645219.1 C40 family peptidase -
BE930_RS12980 2052878..2053138 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T71555 WP_001263491.1 NZ_CP019029:c2047878-2047480 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T71555 NZ_CP019029:c2047878-2047480 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT71555 WP_000554755.1 NZ_CP019029:c2048174-2047881 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT71555 NZ_CP019029:c2048174-2047881 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References