Detailed information of TA system TA071538

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 143562..143983
• Replicon: plasmid pEC881_1
• Accession: NZ_CP019028
• Organism: Escherichia coli strain Ecol_881

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BE930_RS02165
• Protein ID: WP_001302184.1
• Coordinates: 143825..143983 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	143562..143760 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE930_RS02135	139258..140652	+	1395	WP_001037799.1	carbohydrate porin	-
BE930_RS29250	140898..141002	-	105	Protein_159	thiosulfate reductase cytochrome B subunit	-
BE930_RS02140	141059..141752	+	694	Protein_160	IS1 family transposase	-
BE930_RS02145	141768..142391	+	624	WP_000912556.1	potassium channel family protein	-
BE930_RS02150	142476..142727	+	252	Protein_162	iron-containing alcohol dehydrogenase	-
BE930_RS02155	143016..143333	-	318	Protein_163	IS110 family transposase	-
BE930_RS02160	143488..143593	+	106	Protein_164	plasmid SOS inhibition protein A	-
-	143562..143760	+	199	NuclAT_0	-	Antitoxin
-	143562..143760	+	199	NuclAT_0	-	Antitoxin
-	143562..143760	+	199	NuclAT_0	-	Antitoxin
-	143562..143760	+	199	NuclAT_0	-	Antitoxin
BE930_RS02165	143825..143983	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE930_RS29700	144651..144857	+	207	WP_000275854.1	hypothetical protein	-
BE930_RS02180	144882..145169	+	288	WP_000107242.1	hypothetical protein	-
BE930_RS02185	145288..146109	+	822	WP_001234465.1	DUF945 domain-containing protein	-
BE930_RS02190	146405..146977	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..147346	147346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T71538 WP_001302184.1 NZ_CP019028:143825-143983 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71538 NZ_CP019028:143825-143983 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT71538 NZ_CP019028:143562-143760 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin