Detailed information of TA system TA071532

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35633..35887
• Replicon: plasmid pEC881_1
• Accession: NZ_CP019028
• Organism: Escherichia coli strain Ecol_881

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BE930_RS01420
• Protein ID: WP_015387399.1
• Coordinates: 35633..35839 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	35826..35887 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE930_RS01365	30669..31415	+	747	WP_000205701.1	conjugal transfer pilus acetylase TraX	-
BE930_RS01370	31470..32030	+	561	WP_000139363.1	fertility inhibition protein FinO	-
BE930_RS01375	32163..32375	+	213	WP_005012601.1	hypothetical protein	-
BE930_RS01385	32676..33382	-	707	Protein_37	IS1 family transposase	-
BE930_RS01395	33915..34397	+	483	Protein_38	VENN motif pre-toxin domain-containing protein	-
BE930_RS01400	34343..34789	-	447	Protein_39	incFII family plasmid replication initiator RepA	-
BE930_RS01405	34782..34856	-	75	WP_001365705.1	RepA leader peptide Tap	-
BE930_RS01415	35092..35349	-	258	WP_000083833.1	replication regulatory protein RepA	-
BE930_RS01420	35633..35839	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	35826..35887	+	62	NuclAT_1	-	Antitoxin
-	35826..35887	+	62	NuclAT_1	-	Antitoxin
-	35826..35887	+	62	NuclAT_1	-	Antitoxin
-	35826..35887	+	62	NuclAT_1	-	Antitoxin
BE930_RS01430	36026..36208	-	183	WP_000968309.1	hypothetical protein	-
BE930_RS01440	36309..37015	+	707	Protein_44	IS1 family transposase	-
BE930_RS01445	37455..38675	+	1221	WP_000410951.1	arginine deiminase	-
BE930_RS01450	38686..39597	+	912	WP_000440183.1	carbamate kinase	-
BE930_RS01455	39682..40686	+	1005	WP_000154544.1	ornithine carbamoyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..147346	147346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T71532 WP_015387399.1 NZ_CP019028:c35839-35633 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71532 NZ_CP019028:c35839-35633 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71532 NZ_CP019028:35826-35887 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin