Detailed information of TA system TA071530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4183959..4184561
• Replicon: chromosome
• Accession: NZ_CP019020
• Organism: Escherichia coli strain Ecol_244

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: BE937_RS24255
• Protein ID: WP_000897302.1
• Coordinates: 4184250..4184561 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE937_RS24250
• Protein ID: WP_000356397.1
• Coordinates: 4183959..4184249 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE937_RS24210	4179266..4180195	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
BE937_RS24215	4180377..4180619	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
BE937_RS24220	4180909..4181757	+	849	WP_001038650.1	hypothetical protein	-
BE937_RS24225	4182073..4182522	+	450	WP_032140890.1	hypothetical protein	-
BE937_RS24230	4182707..4182925	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
BE937_RS24240	4183322..4183600	-	279	WP_001296612.1	hypothetical protein	-
BE937_RS24250	4183959..4184249	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BE937_RS24255	4184250..4184561	-	312	WP_000897302.1	hypothetical protein	Toxin
BE937_RS24260	4184790..4185698	+	909	WP_001385591.1	alpha/beta hydrolase	-
BE937_RS24265	4185762..4186703	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE937_RS24270	4186748..4187185	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BE937_RS24275	4187182..4188054	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BE937_RS24280	4188048..4188647	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T71530 WP_000897302.1 NZ_CP019020:c4184561-4184250 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71530 NZ_CP019020:c4184561-4184250 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71530 WP_000356397.1 NZ_CP019020:c4184249-4183959 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71530 NZ_CP019020:c4184249-4183959 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416