Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1330157..1330659 Replicon chromosome
Accession NZ_CP019020
Organism Escherichia coli strain Ecol_244

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7A2Z7A8
Locus tag BE937_RS09280 Protein ID WP_024705227.1
Coordinates 1330405..1330659 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BE937_RS09275 Protein ID WP_001259255.1
Coordinates 1330157..1330408 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE937_RS09245 1325335..1326402 - 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BE937_RS09250 1326402..1327472 - 1071 WP_061157916.1 histidinol-phosphate transaminase -
BE937_RS09255 1327469..1328773 - 1305 WP_061157915.1 histidinol dehydrogenase -
BE937_RS09260 1328779..1329678 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
BE937_RS09270 1329824..1329874 - 51 WP_001364200.1 his operon leader peptide -
BE937_RS09275 1330157..1330408 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BE937_RS09280 1330405..1330659 + 255 WP_024705227.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BE937_RS09285 1330742..1331566 + 825 WP_000754737.1 SDR family oxidoreductase -
BE937_RS09290 1331612..1332541 + 930 WP_000803366.1 LysR family transcriptional regulator -
BE937_RS28130 1332756..1332818 + 63 WP_010723108.1 membrane protein YoeI -
BE937_RS09295 1332808..1334166 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T71513 WP_024705227.1 NZ_CP019020:1330405-1330659 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71513 NZ_CP019020:1330405-1330659 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT71513 WP_001259255.1 NZ_CP019020:1330157-1330408 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT71513 NZ_CP019020:1330157-1330408 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACCATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7A2Z7A8

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References