Detailed information of TA system TA071504

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108286..108712
• Replicon: plasmid pEC244_2
• Accession: NZ_CP019018
• Organism: Escherichia coli strain Ecol_244

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BE937_RS01350
• Protein ID: WP_001302184.1
• Coordinates: 108286..108444 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	108488..108712 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE937_RS01315	103459..103854	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
BE937_RS01320	103953..104642	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
BE937_RS01325	104829..105212	-	384	WP_001151524.1	relaxosome protein TraM	-
BE937_RS01330	105533..106135	+	603	WP_077250849.1	transglycosylase SLT domain-containing protein	-
BE937_RS01335	106432..107253	-	822	WP_001234487.1	DUF945 domain-containing protein	-
BE937_RS01340	107364..107660	-	297	WP_001272245.1	hypothetical protein	-
BE937_RS01350	108286..108444	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	108488..108712	-	225	NuclAT_0	-	Antitoxin
-	108488..108712	-	225	NuclAT_0	-	Antitoxin
-	108488..108712	-	225	NuclAT_0	-	Antitoxin
-	108488..108712	-	225	NuclAT_0	-	Antitoxin
BE937_RS01355	108724..109443	-	720	WP_001276236.1	plasmid SOS inhibition protein A	-
BE937_RS01360	109440..109874	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
BE937_RS01365	109943..111907	-	1965	WP_001593049.1	ParB/RepB/Spo0J family partition protein	-
BE937_RS01370	111966..112199	-	234	WP_000005985.1	DUF905 family protein	-
BE937_RS01375	112257..112796	-	540	WP_001593051.1	single-stranded DNA-binding protein	-
BE937_RS28615	112822..113028	-	207	WP_000547969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158911	158911
	-	flank	IS/Tn	-	-	113349..114320	971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T71504 WP_001302184.1 NZ_CP019018:c108444-108286 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71504 NZ_CP019018:c108444-108286 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT71504 NZ_CP019018:c108712-108488 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin