Detailed information of TA system TA071499

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70178..70432
• Replicon: plasmid pEC244_2
• Accession: NZ_CP019018
• Organism: Escherichia coli strain Ecol_244

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BE937_RS01125
• Protein ID: WP_001351576.1
• Coordinates: 70178..70384 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70371..70432 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE937_RS01075	65431..66546	+	1116	Protein_59	IS91 family transposase	-
BE937_RS01085	66727..67071	-	345	WP_001553768.1	hypothetical protein	-
BE937_RS28465	67163..67303	-	141	WP_161956652.1	hypothetical protein	-
BE937_RS01100	68006..68863	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
BE937_RS01105	68856..69338	-	483	WP_001273588.1	hypothetical protein	-
BE937_RS01110	69331..69405	-	75	WP_001442103.1	RepA leader peptide Tap	-
BE937_RS01120	69637..69894	-	258	WP_000083845.1	replication regulatory protein RepA	-
BE937_RS01125	70178..70384	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	70371..70432	+	62	NuclAT_1	-	Antitoxin
-	70371..70432	+	62	NuclAT_1	-	Antitoxin
-	70371..70432	+	62	NuclAT_1	-	Antitoxin
-	70371..70432	+	62	NuclAT_1	-	Antitoxin
BE937_RS28470	70688..70762	-	75	Protein_67	endonuclease	-
BE937_RS01135	71008..71220	-	213	WP_001299730.1	ANR family transcriptional regulator	-
BE937_RS01140	71356..71916	-	561	WP_000139315.1	fertility inhibition protein FinO	-
BE937_RS01145	72019..72879	-	861	WP_000704513.1	alpha/beta hydrolase	-
BE937_RS01150	72938..73684	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158911	158911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T71499 WP_001351576.1 NZ_CP019018:c70384-70178 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71499 NZ_CP019018:c70384-70178 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71499 NZ_CP019018:70371-70432 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin