Detailed information of TA system TA071483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3821401..3821903
• Replicon: chromosome
• Accession: NZ_CP019015
• Organism: Escherichia coli strain Ecol_AZ162

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: BE965_RS20850
• Protein ID: WP_000767819.1
• Coordinates: 3821649..3821903 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: BE965_RS20845
• Protein ID: WP_001259253.1
• Coordinates: 3821401..3821652 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE965_RS20815	3816579..3817646	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
BE965_RS20820	3817646..3818716	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
BE965_RS20825	3818713..3820017	-	1305	WP_001296211.1	histidinol dehydrogenase	-
BE965_RS20830	3820023..3820922	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
BE965_RS20840	3821068..3821118	-	51	WP_001364200.1	his operon leader peptide	-
BE965_RS20845	3821401..3821652	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
BE965_RS20850	3821649..3821903	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
BE965_RS20855	3821986..3822810	+	825	WP_000754737.1	SDR family oxidoreductase	-
BE965_RS20860	3822856..3823785	+	930	WP_000803366.1	LysR family transcriptional regulator	-
BE965_RS28430	3824000..3824062	+	63	WP_010723108.1	membrane protein YoeI	-
BE965_RS20865	3824052..3825410	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
BE965_RS20875	3825627..3826085	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3825627..3826085	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T71483 WP_000767819.1 NZ_CP019015:3821649-3821903 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T71483 NZ_CP019015:3821649-3821903 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT71483 WP_001259253.1 NZ_CP019015:3821401-3821652 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT71483 NZ_CP019015:3821401-3821652 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3