Detailed information of TA system TA071464

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106876..107145
• Replicon: plasmid pECAZ162_KPC
• Accession: NZ_CP019014
• Organism: Escherichia coli strain Ecol_AZ162

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE965_RS01375
• Protein ID: WP_001312861.1
• Coordinates: 106987..107145 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	106876..106941 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE965_RS01350	102669..103196	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
BE965_RS01355	103252..103485	+	234	WP_000006004.1	DUF905 domain-containing protein	-
BE965_RS01360	103544..105502	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
BE965_RS01365	105557..105991	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
BE965_RS01370	105988..106707	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
BE965_RS28630	106719..106907	-	189	WP_001299721.1	hypothetical protein	-
-	106719..106943	+	225	NuclAT_0	-	-
-	106719..106943	+	225	NuclAT_0	-	-
-	106719..106943	+	225	NuclAT_0	-	-
-	106719..106943	+	225	NuclAT_0	-	-
-	106876..106941	+	66	-	-	Antitoxin
BE965_RS01375	106987..107145	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE965_RS01395	108064..108351	+	288	WP_000107537.1	hypothetical protein	-
BE965_RS01400	108472..109293	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BE965_RS01405	109590..110237	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
BE965_RS01410	110523..110906	+	384	WP_001151564.1	relaxosome protein TraM	-
BE965_RS01415	111100..111786	+	687	WP_000332487.1	PAS domain-containing protein	-
BE965_RS01420	111880..112107	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / blaKPC-3 / tet(A) / sul1 / qacE / aadA5 / sul2	-	1..142829	142829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71464 WP_001312861.1 NZ_CP019014:106987-107145 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71464 NZ_CP019014:106987-107145 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT71464 NZ_CP019014:106876-106941 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin