Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4157811..4158313 Replicon chromosome
Accession NZ_CP019012
Organism Escherichia coli strain Ecol_AZ161

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag BE946_RS22885 Protein ID WP_000767819.1
Coordinates 4158059..4158313 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag BE946_RS22880 Protein ID WP_001259253.1
Coordinates 4157811..4158062 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE946_RS22850 4152989..4154056 - 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BE946_RS22855 4154056..4155126 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
BE946_RS22860 4155123..4156427 - 1305 WP_001296211.1 histidinol dehydrogenase -
BE946_RS22865 4156433..4157332 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BE946_RS22875 4157478..4157528 - 51 WP_001364200.1 his operon leader peptide -
BE946_RS22880 4157811..4158062 + 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BE946_RS22885 4158059..4158313 + 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BE946_RS22890 4158396..4159220 + 825 WP_000754737.1 SDR family oxidoreductase -
BE946_RS22895 4159266..4160195 + 930 WP_000803366.1 LysR family transcriptional regulator -
BE946_RS27460 4160410..4160472 + 63 WP_010723108.1 membrane protein YoeI -
BE946_RS22900 4160462..4161820 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -
BE946_RS22910 4162037..4162495 + 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T71448 WP_000767819.1 NZ_CP019012:4158059-4158313 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71448 NZ_CP019012:4158059-4158313 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT71448 WP_001259253.1 NZ_CP019012:4157811-4158062 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp

>AT71448 NZ_CP019012:4157811-4158062 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References