Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2907470..2908269 Replicon chromosome
Accession NZ_CP019012
Organism Escherichia coli strain Ecol_AZ161

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag BE946_RS16795 Protein ID WP_000347251.1
Coordinates 2907470..2907934 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag BE946_RS16800 Protein ID WP_001296435.1
Coordinates 2907934..2908269 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE946_RS16760 2902471..2902905 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
BE946_RS16765 2902923..2903801 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
BE946_RS16770 2903791..2904570 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
BE946_RS16775 2904581..2905054 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
BE946_RS16780 2905077..2906357 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
BE946_RS16790 2906606..2907415 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
BE946_RS16795 2907470..2907934 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
BE946_RS16800 2907934..2908269 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
BE946_RS16805 2908418..2909989 - 1572 WP_001273763.1 galactarate dehydratase -
BE946_RS16810 2910364..2911698 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
BE946_RS16815 2911714..2912484 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T71444 WP_000347251.1 NZ_CP019012:c2907934-2907470 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T71444 NZ_CP019012:c2907934-2907470 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT71444 WP_001296435.1 NZ_CP019012:c2908269-2907934 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT71444 NZ_CP019012:c2908269-2907934 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References