Detailed information of TA system TA071442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2094455..2095057
• Replicon: chromosome
• Accession: NZ_CP019012
• Organism: Escherichia coli strain Ecol_AZ161

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: BE946_RS12655
• Protein ID: WP_000897302.1
• Coordinates: 2094746..2095057 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE946_RS12650
• Protein ID: WP_000356397.1
• Coordinates: 2094455..2094745 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE946_RS12610	2089762..2090691	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
BE946_RS12615	2090873..2091115	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
BE946_RS12620	2091405..2092253	+	849	WP_001038650.1	hypothetical protein	-
BE946_RS12625	2092569..2093018	+	450	WP_032140890.1	hypothetical protein	-
BE946_RS12630	2093203..2093421	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
BE946_RS12640	2093818..2094096	-	279	WP_001296612.1	hypothetical protein	-
BE946_RS12650	2094455..2094745	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BE946_RS12655	2094746..2095057	-	312	WP_000897302.1	hypothetical protein	Toxin
BE946_RS12660	2095286..2096194	+	909	WP_001385591.1	alpha/beta hydrolase	-
BE946_RS12665	2096258..2097199	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE946_RS12670	2097244..2097681	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BE946_RS12675	2097678..2098550	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BE946_RS12680	2098544..2099143	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T71442 WP_000897302.1 NZ_CP019012:c2095057-2094746 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71442 NZ_CP019012:c2095057-2094746 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71442 WP_000356397.1 NZ_CP019012:c2094745-2094455 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71442 NZ_CP019012:c2094745-2094455 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416