Detailed information of TA system TA071427

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65786..66028
• Replicon: plasmid pECAZ161_3
• Accession: NZ_CP019009
• Organism: Escherichia coli strain Ecol_AZ161

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE946_RS00430
• Protein ID: WP_001312861.1
• Coordinates: 65786..65944 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65988..66028 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE946_RS00380	60898..61125	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
BE946_RS00385	61213..61890	-	678	WP_001348626.1	PAS domain-containing protein	-
BE946_RS00390	62024..62407	-	384	WP_001151566.1	relaxosome protein TraM	-
BE946_RS00395	62738..63340	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BE946_RS00400	63637..64458	-	822	WP_001234469.1	DUF945 domain-containing protein	-
BE946_RS00405	64577..64864	-	288	WP_000107535.1	hypothetical protein	-
BE946_RS27800	64889..65095	-	207	WP_000275859.1	hypothetical protein	-
BE946_RS00430	65786..65944	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65988..66028	-	41	NuclAT_1	-	Antitoxin
-	65988..66028	-	41	NuclAT_1	-	Antitoxin
-	65988..66028	-	41	NuclAT_1	-	Antitoxin
-	65988..66028	-	41	NuclAT_1	-	Antitoxin
-	67472..67658	-	187	NuclAT_0	-	-
-	67472..67658	-	187	NuclAT_0	-	-
-	67472..67658	-	187	NuclAT_0	-	-
-	67472..67658	-	187	NuclAT_0	-	-
BE946_RS00445	67670..68389	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
BE946_RS00450	68386..68820	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
BE946_RS00455	68875..70833	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / tet(A) / aac(3)-IId / blaTEM-1B / dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2	-	1..130905	130905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71427 WP_001312861.1 NZ_CP019009:c65944-65786 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71427 NZ_CP019009:c65944-65786 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT71427 NZ_CP019009:c66028-65988 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin