Detailed information of TA system TA071394

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 9415..9684
• Replicon: plasmid pECAZ159_1
• Accession: NZ_CP019007
• Organism: Escherichia coli strain Ecol_AZ159

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE939_RS00420
• Protein ID: WP_001312861.1
• Coordinates: 9526..9684 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	9415..9480 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE939_RS26665	4463..4735	+	273	WP_072133095.1	hypothetical protein	-
BE939_RS00395	5208..5735	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
BE939_RS00400	5791..6024	+	234	WP_000006004.1	DUF905 domain-containing protein	-
BE939_RS00405	6083..8041	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
BE939_RS00410	8096..8530	+	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
BE939_RS00415	8527..9246	+	720	WP_001276228.1	plasmid SOS inhibition protein A	-
BE939_RS26265	9258..9446	-	189	WP_001299721.1	hypothetical protein	-
-	9258..9482	+	225	NuclAT_0	-	-
-	9258..9482	+	225	NuclAT_0	-	-
-	9258..9482	+	225	NuclAT_0	-	-
-	9258..9482	+	225	NuclAT_0	-	-
-	9415..9480	+	66	-	-	Antitoxin
BE939_RS00420	9526..9684	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE939_RS26670	10375..10581	+	207	WP_000547939.1	hypothetical protein	-
BE939_RS00445	10606..10893	+	288	WP_078232962.1	hypothetical protein	-
BE939_RS00450	11014..11835	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BE939_RS00455	12132..12734	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
BE939_RS00460	13055..13438	+	384	WP_001151524.1	relaxosome protein TraM	-
BE939_RS00465	13625..14314	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroN / iroE / iroD / iroC / iroB	1..128248	128248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71394 WP_001312861.1 NZ_CP019007:9526-9684 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71394 NZ_CP019007:9526-9684 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT71394 NZ_CP019007:9415-9480 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin