Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3468271..3468773 Replicon chromosome
Accession NZ_CP019005
Organism Escherichia coli strain Ecol_AZ155

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BE948_RS20650 Protein ID WP_000767829.1
Coordinates 3468271..3468525 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BE948_RS20655 Protein ID WP_001259255.1
Coordinates 3468522..3468773 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE948_RS20625 3463286..3463513 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BE948_RS20630 3463527..3464585 - 1059 WP_000492321.1 transport protein YeeE -
BE948_RS20635 3464764..3466122 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BE948_RS27175 3466112..3466174 - 63 WP_010723108.1 membrane protein YoeI -
BE948_RS20640 3466389..3467318 - 930 WP_000803351.1 LysR family transcriptional regulator -
BE948_RS20645 3467364..3468188 - 825 WP_000754749.1 SDR family oxidoreductase -
BE948_RS20650 3468271..3468525 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BE948_RS20655 3468522..3468773 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BE948_RS20660 3469057..3469107 + 51 WP_001364200.1 his operon leader peptide -
BE948_RS20670 3469253..3470152 + 900 WP_032329340.1 ATP phosphoribosyltransferase -
BE948_RS20675 3470158..3471462 + 1305 WP_001446927.1 histidinol dehydrogenase -
BE948_RS20680 3471459..3472529 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
BE948_RS20685 3472529..3473596 + 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T71388 WP_000767829.1 NZ_CP019005:c3468525-3468271 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71388 NZ_CP019005:c3468525-3468271 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT71388 WP_001259255.1 NZ_CP019005:c3468773-3468522 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT71388 NZ_CP019005:c3468773-3468522 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References