Detailed information of TA system TA071368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 139462..140119
• Replicon: plasmid pECAZ155_KPC
• Accession: NZ_CP019001
• Organism: Escherichia coli strain Ecol_AZ155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: BE948_RS00990
• Protein ID: WP_000270043.1
• Coordinates: 139769..140119 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE948_RS00985
• Protein ID: WP_000124640.1
• Coordinates: 139462..139764 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE948_RS00935	135087..135515	+	429	WP_000591074.1	hypothetical protein	-
BE948_RS00940	135572..135931	+	360	WP_000422768.1	hypothetical protein	-
BE948_RS00945	135931..136377	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
BE948_RS00950	136374..136892	+	519	WP_000210756.1	nitrite reductase	-
BE948_RS00955	136892..137122	+	231	WP_000972663.1	hypothetical protein	-
BE948_RS00960	137109..137966	+	858	WP_001167032.1	hypothetical protein	-
BE948_RS00965	137992..138180	+	189	WP_001270411.1	hypothetical protein	-
BE948_RS00970	138197..138724	+	528	WP_001236377.1	thermonuclease family protein	-
BE948_RS00975	138782..139054	+	273	WP_001043047.1	HU family DNA-binding protein	-
BE948_RS00980	139142..139435	+	294	WP_001239997.1	chromosome segregation protein ParM	-
BE948_RS00985	139462..139764	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
BE948_RS00990	139769..140119	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE948_RS00995	140282..140830	+	549	WP_001061574.1	transcriptional regulator	-
BE948_RS01005	141171..141365	+	195	WP_000343597.1	hypothetical protein	-
BE948_RS01010	141376..141747	+	372	WP_000516916.1	hypothetical protein	-
BE948_RS01015	141740..142210	+	471	WP_001281821.1	hypothetical protein	-
BE948_RS01020	142225..142560	-	336	WP_000683476.1	hypothetical protein	-
BE948_RS01025	142657..143145	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
BE948_RS01030	143148..143645	+	498	WP_000062185.1	hypothetical protein	-
BE948_RS01040	143860..144564	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BE948_RS01045	144629..144808	-	180	Protein_181	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / sul1 / qacE / aac(6')-Il / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / tet(B) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..272202	272202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T71368 WP_000270043.1 NZ_CP019001:c140119-139769 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T71368 NZ_CP019001:c140119-139769 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT71368 WP_000124640.1 NZ_CP019001:c139764-139462 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT71368 NZ_CP019001:c139764-139462 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9