Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1212286..1212788 Replicon chromosome
Accession NZ_CP018995
Organism Escherichia coli strain Ecol_AZ147

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BE962_RS08445 Protein ID WP_000767829.1
Coordinates 1212534..1212788 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BE962_RS08440 Protein ID WP_001259255.1
Coordinates 1212286..1212537 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE962_RS08410 1207463..1208530 - 1068 WP_078232713.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BE962_RS08415 1208530..1209600 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
BE962_RS08420 1209597..1210901 - 1305 WP_001446927.1 histidinol dehydrogenase -
BE962_RS08425 1210907..1211806 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BE962_RS08435 1211952..1212002 - 51 WP_001364200.1 his operon leader peptide -
BE962_RS08440 1212286..1212537 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BE962_RS08445 1212534..1212788 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BE962_RS08450 1212871..1213695 + 825 WP_000754737.1 SDR family oxidoreductase -
BE962_RS08455 1213741..1214670 + 930 WP_000803366.1 LysR family transcriptional regulator -
BE962_RS26820 1214885..1214947 + 63 WP_010723108.1 membrane protein YoeI -
BE962_RS08460 1214937..1216295 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BE962_RS08465 1216475..1217533 + 1059 WP_000492330.1 transport protein YeeE -
BE962_RS08470 1217547..1217774 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T71309 WP_000767829.1 NZ_CP018995:1212534-1212788 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71309 NZ_CP018995:1212534-1212788 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT71309 WP_001259255.1 NZ_CP018995:1212286-1212537 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT71309 NZ_CP018995:1212286-1212537 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References