Detailed information of TA system TA071299

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91966..92230
• Replicon: plasmid pECAZ147_2
• Accession: NZ_CP018993
• Organism: Escherichia coli strain Ecol_AZ147

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: BE962_RS01275
• Protein ID: WP_001331364.1
• Coordinates: 91966..92118 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	92173..92230 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE962_RS01255	87300..89468	+	2169	WP_078232638.1	DotA/TraY family protein	-
BE962_RS01260	89533..90195	+	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
BE962_RS01265	90267..90476	-	210	WP_000062602.1	HEAT repeat domain-containing protein	-
BE962_RS27440	90808..90984	+	177	WP_001054900.1	hypothetical protein	-
BE962_RS01270	91643..91894	+	252	WP_001291964.1	hypothetical protein	-
BE962_RS01275	91966..92118	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	92173..92230	+	58	NuclAT_0	-	Antitoxin
-	92173..92230	+	58	NuclAT_0	-	Antitoxin
-	92173..92230	+	58	NuclAT_0	-	Antitoxin
-	92173..92230	+	58	NuclAT_0	-	Antitoxin
BE962_RS01280	92391..93377	+	987	WP_001257838.1	hypothetical protein	-
BE962_RS01285	93595..94803	+	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
BE962_RS01290	94822..95892	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / aac(3)-IVa / aph(4)-Ia / tet(M) / floR	-	1..116562	116562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T71299 WP_001331364.1 NZ_CP018993:c92118-91966 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T71299 NZ_CP018993:c92118-91966 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT71299 NZ_CP018993:92173-92230 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin