Detailed information of TA system TA071281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3111674..3112276
• Replicon: chromosome
• Accession: NZ_CP018991
• Organism: Escherichia coli strain Ecol_AZ146

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: BE932_RS18285
• Protein ID: WP_000897302.1
• Coordinates: 3111674..3111985 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE932_RS18290
• Protein ID: WP_000356397.1
• Coordinates: 3111986..3112276 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE932_RS18260	3107588..3108187	+	600	WP_001296610.1	glucose-1-phosphatase	-
BE932_RS18265	3108181..3109053	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BE932_RS18270	3109050..3109487	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BE932_RS18275	3109532..3110473	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE932_RS18280	3110537..3111445	-	909	WP_001385591.1	alpha/beta hydrolase	-
BE932_RS18285	3111674..3111985	+	312	WP_000897302.1	hypothetical protein	Toxin
BE932_RS18290	3111986..3112276	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BE932_RS18295	3112361..3112573	+	213	WP_000197774.1	hypothetical protein	-
BE932_RS18300	3112635..3112913	+	279	WP_001296612.1	hypothetical protein	-
BE932_RS18310	3113310..3113528	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
BE932_RS18315	3113713..3114162	-	450	WP_032140890.1	hypothetical protein	-
BE932_RS18320	3114478..3115326	-	849	WP_001038650.1	hypothetical protein	-
BE932_RS18325	3115616..3115858	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
BE932_RS18330	3116040..3116969	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T71281 WP_000897302.1 NZ_CP018991:3111674-3111985 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71281 NZ_CP018991:3111674-3111985 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71281 WP_000356397.1 NZ_CP018991:3111986-3112276 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71281 NZ_CP018991:3111986-3112276 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416