Detailed information of TA system TA071261

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93370..93624
• Replicon: plasmid pECAZ146_1
• Accession: NZ_CP018990
• Organism: Escherichia coli strain Ecol_AZ146

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BE932_RS01560
• Protein ID: WP_015387399.1
• Coordinates: 93418..93624 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93370..93431 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE932_RS01510	89022..89234	+	213	WP_005012601.1	hypothetical protein	-
BE932_RS31165	89535..89624	-	90	Protein_105	IS1 family transposase	-
BE932_RS01525	89679..90356	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
BE932_RS01530	90356..90703	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
BE932_RS01535	90723..92294	+	1572	WP_023149734.1	IS66 family transposase	-
BE932_RS01540	92332..92948	-	617	Protein_109	IS1 family transposase	-
BE932_RS01550	93049..93231	+	183	WP_000968309.1	hypothetical protein	-
-	93370..93431	-	62	NuclAT_1	-	Antitoxin
-	93370..93431	-	62	NuclAT_1	-	Antitoxin
-	93370..93431	-	62	NuclAT_1	-	Antitoxin
-	93370..93431	-	62	NuclAT_1	-	Antitoxin
BE932_RS01560	93418..93624	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE932_RS01565	93908..94165	+	258	WP_000083833.1	replication regulatory protein RepA	-
BE932_RS01575	94401..94475	+	75	WP_032336874.1	RepA leader peptide Tap	-
BE932_RS01580	94468..94914	+	447	Protein_114	incFII family plasmid replication initiator RepA	-
BE932_RS01585	94860..95342	-	483	Protein_115	VENN motif pre-toxin domain-containing protein	-
BE932_RS01590	96235..97455	+	1221	WP_000410951.1	arginine deiminase	-
BE932_RS01595	97466..98377	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / aadA5	iucD / iutA	1..150994	150994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T71261 WP_015387399.1 NZ_CP018990:93418-93624 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71261 NZ_CP018990:93418-93624 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71261 NZ_CP018990:c93431-93370 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin