Detailed information of TA system TA071258

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53270..53539
• Replicon: plasmid pECAZ146_1
• Accession: NZ_CP018990
• Organism: Escherichia coli strain Ecol_AZ146

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE932_RS01300
• Protein ID: WP_001312861.1
• Coordinates: 53381..53539 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53270..53335 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE932_RS01270	48822..50723	+	1902	WP_000936285.1	group II intron reverse transcriptase/maturase	-
BE932_RS01275	50873..51370	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
BE932_RS01280	51438..51671	+	234	WP_000005990.1	DUF905 family protein	-
BE932_RS01285	51699..51896	+	198	Protein_61	hypothetical protein	-
BE932_RS01290	51951..52385	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
BE932_RS01295	52382..53101	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
BE932_RS30595	53113..53301	-	189	WP_001299721.1	hypothetical protein	-
-	53113..53337	+	225	NuclAT_0	-	-
-	53113..53337	+	225	NuclAT_0	-	-
-	53113..53337	+	225	NuclAT_0	-	-
-	53113..53337	+	225	NuclAT_0	-	-
-	53270..53335	+	66	-	-	Antitoxin
BE932_RS01300	53381..53539	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE932_RS01320	54460..54747	+	288	WP_000107535.1	hypothetical protein	-
BE932_RS01325	54865..55686	+	822	WP_001234426.1	DUF945 domain-containing protein	-
BE932_RS01330	55983..56585	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
BE932_RS01335	56906..57289	+	384	WP_001151524.1	relaxosome protein TraM	-
BE932_RS01340	57476..58165	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / aadA5	iucD / iutA	1..150994	150994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71258 WP_001312861.1 NZ_CP018990:53381-53539 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71258 NZ_CP018990:53381-53539 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT71258 NZ_CP018990:53270-53335 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin