Detailed information of TA system TA071222

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61682..61921
• Replicon: plasmid pEC867_1
• Accession: NZ_CP018982
• Organism: Escherichia coli strain Ecol_867

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: BE928_RS00485
• Protein ID: WP_023144756.1
• Coordinates: 61787..61921 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	61682..61742 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE928_RS00450	57473..58033	+	561	WP_000139341.1	fertility inhibition protein FinO	-
BE928_RS00455	58164..58376	+	213	WP_013023861.1	hypothetical protein	-
BE928_RS00465	58935..59360	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
BE928_RS00470	59357..59707	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
BE928_RS00475	59738..61351	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
BE928_RS00480	61429..61715	+	287	Protein_64	DUF2726 domain-containing protein	-
-	61682..61742	-	61	NuclAT_2	-	Antitoxin
-	61682..61742	-	61	NuclAT_2	-	Antitoxin
-	61682..61742	-	61	NuclAT_2	-	Antitoxin
-	61682..61742	-	61	NuclAT_2	-	Antitoxin
BE928_RS00485	61787..61921	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE928_RS00490	62218..62472	+	255	WP_000083850.1	replication regulatory protein RepA	-
BE928_RS27065	62578..62712	+	135	Protein_67	protein CopA/IncA	-
BE928_RS00500	62709..62783	+	75	WP_031943482.1	RepA leader peptide Tap	-
BE928_RS00505	62776..63633	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
BE928_RS00515	64573..65226	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BE928_RS26845	65319..65576	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BE928_RS00520	65509..65910	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..100168	100168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T71222 WP_023144756.1 NZ_CP018982:61787-61921 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T71222 NZ_CP018982:61787-61921 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT71222 NZ_CP018982:c61742-61682 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin