Detailed information of TA system TA071218

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41837..42079
• Replicon: plasmid pEC867_1
• Accession: NZ_CP018982
• Organism: Escherichia coli strain Ecol_867

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE928_RS00365
• Protein ID: WP_001312861.1
• Coordinates: 41921..42079 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41837..41877 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE928_RS00340	37032..38990	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
BE928_RS00345	39045..39479	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
BE928_RS00350	39476..40195	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	40207..40393	+	187	NuclAT_0	-	-
-	40207..40393	+	187	NuclAT_0	-	-
-	40207..40393	+	187	NuclAT_0	-	-
-	40207..40393	+	187	NuclAT_0	-	-
BE928_RS00360	40441..41810	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	41837..41877	+	41	NuclAT_1	-	Antitoxin
-	41837..41877	+	41	NuclAT_1	-	Antitoxin
-	41837..41877	+	41	NuclAT_1	-	Antitoxin
-	41837..41877	+	41	NuclAT_1	-	Antitoxin
BE928_RS00365	41921..42079	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE928_RS27055	42522..42857	+	336	WP_013023876.1	hypothetical protein	-
BE928_RS27060	42770..42976	+	207	WP_000275859.1	hypothetical protein	-
BE928_RS00390	43001..43288	+	288	WP_000107535.1	hypothetical protein	-
BE928_RS00395	43407..44228	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BE928_RS00400	44525..45127	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BE928_RS00405	45458..45841	+	384	WP_001151566.1	relaxosome protein TraM	-
BE928_RS00410	45975..46406	+	432	WP_078214978.1	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..100168	100168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71218 WP_001312861.1 NZ_CP018982:41921-42079 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71218 NZ_CP018982:41921-42079 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT71218 NZ_CP018982:41837-41877 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin