Detailed information of TA system TA071181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51629..51899
• Replicon: plasmid pEC656_1
• Accession: NZ_CP018978
• Organism: Escherichia coli strain Ecol_656

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE941_RS00750
• Protein ID: WP_001312861.1
• Coordinates: 51741..51899 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51629..51692 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE941_RS00725	47423..47950	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
BE941_RS00730	48006..48239	+	234	WP_000006004.1	DUF905 domain-containing protein	-
BE941_RS00735	48298..50256	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
BE941_RS00740	50311..50745	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
BE941_RS00745	50742..51461	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
BE941_RS28425	51473..51661	-	189	WP_001299721.1	hypothetical protein	-
-	51473..51697	+	225	NuclAT_0	-	-
-	51473..51697	+	225	NuclAT_0	-	-
-	51473..51697	+	225	NuclAT_0	-	-
-	51473..51697	+	225	NuclAT_0	-	-
-	51629..51692	-	64	-	-	Antitoxin
BE941_RS00750	51741..51899	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE941_RS00770	52818..53105	+	288	WP_000107537.1	hypothetical protein	-
BE941_RS00775	53226..54047	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BE941_RS00780	54344..54991	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
BE941_RS00785	55277..55660	+	384	WP_001151564.1	relaxosome protein TraM	-
BE941_RS00790	55854..56540	+	687	WP_000332487.1	PAS domain-containing protein	-
BE941_RS00795	56634..56861	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A)	-	1..117844	117844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71181 WP_001312861.1 NZ_CP018978:51741-51899 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71181 NZ_CP018978:51741-51899 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT71181 NZ_CP018978:c51692-51629 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin