Detailed information of TA system TA071172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3603509..3604111
• Replicon: chromosome
• Accession: NZ_CP018976
• Organism: Escherichia coli strain Ecol_545

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BE949_RS20090
• Protein ID: WP_000897305.1
• Coordinates: 3603800..3604111 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE949_RS20085
• Protein ID: WP_000356395.1
• Coordinates: 3603509..3603799 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE949_RS20050	3599133..3600035	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BE949_RS20055	3600032..3600667	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BE949_RS20060	3600664..3601593	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BE949_RS20065	3601775..3602017	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
BE949_RS20070	3602236..3602454	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
BE949_RS20075	3602873..3603151	-	279	WP_001315112.1	hypothetical protein	-
BE949_RS20080	3603203..3603424	-	222	WP_001550354.1	hypothetical protein	-
BE949_RS20085	3603509..3603799	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
BE949_RS20090	3603800..3604111	-	312	WP_000897305.1	hypothetical protein	Toxin
BE949_RS20095	3604340..3605248	+	909	WP_001550353.1	alpha/beta hydrolase	-
BE949_RS20105	3605416..3607230	-	1815	WP_001550352.1	hypothetical protein	-
BE949_RS20110	3607646..3608587	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE949_RS20115	3608632..3609069	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T71172 WP_000897305.1 NZ_CP018976:c3604111-3603800 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71172 NZ_CP018976:c3604111-3603800 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT71172 WP_000356395.1 NZ_CP018976:c3603799-3603509 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71172 NZ_CP018976:c3603799-3603509 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7