Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1002244..1002746 Replicon chromosome
Accession NZ_CP018976
Organism Escherichia coli strain Ecol_545

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BE949_RS07055 Protein ID WP_000767829.1
Coordinates 1002244..1002498 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BE949_RS07060 Protein ID WP_001259255.1
Coordinates 1002495..1002746 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE949_RS07030 997259..997486 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BE949_RS07035 997500..998558 - 1059 WP_000492315.1 transport protein YeeE -
BE949_RS07040 998737..1000095 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BE949_RS27470 1000085..1000147 - 63 WP_010723108.1 membrane protein YoeI -
BE949_RS07045 1000362..1001291 - 930 WP_000803351.1 LysR family transcriptional regulator -
BE949_RS07050 1001337..1002161 - 825 WP_000754749.1 SDR family oxidoreductase -
BE949_RS07055 1002244..1002498 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BE949_RS07060 1002495..1002746 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BE949_RS07065 1003029..1003079 + 51 WP_001364200.1 his operon leader peptide -
BE949_RS07075 1003225..1004124 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BE949_RS07080 1004130..1005434 + 1305 WP_042031741.1 histidinol dehydrogenase -
BE949_RS07085 1005431..1006501 + 1071 WP_001682999.1 histidinol-phosphate transaminase -
BE949_RS07090 1006501..1007568 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T71162 WP_000767829.1 NZ_CP018976:c1002498-1002244 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T71162 NZ_CP018976:c1002498-1002244 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT71162 WP_001259255.1 NZ_CP018976:c1002746-1002495 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT71162 NZ_CP018976:c1002746-1002495 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References