Detailed information of TA system TA071147

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 227693..228064
• Replicon: chromosome
• Accession: NZ_CP018976
• Organism: Escherichia coli strain Ecol_545

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: BE949_RS02870
• Protein ID: WP_001317028.1
• Coordinates: 227870..228064 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	227693..227871 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE949_RS02845	223648..225021	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
BE949_RS02850	225150..226085	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
BE949_RS02855	226137..227372	-	1236	WP_072617251.1	site-specific integrase	-
BE949_RS02860	227374..227589	-	216	WP_000079604.1	excisionase XisR	-
BE949_RS02865	227668..227877	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	227693..227871	+	179	NuclAT_0	-	Antitoxin
-	227693..227871	+	179	NuclAT_0	-	Antitoxin
-	227693..227871	+	179	NuclAT_0	-	Antitoxin
-	227693..227871	+	179	NuclAT_0	-	Antitoxin
BE949_RS02870	227870..228064	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
BE949_RS02875	228120..228929	-	810	WP_000166319.1	recombination protein RecT	-
BE949_RS02880	228922..231522	-	2601	WP_072617252.1	exodeoxyribonuclease VIII	-
BE949_RS02885	231622..231897	-	276	WP_001372690.1	hypothetical protein	-
BE949_RS02890	231972..232142	-	171	WP_065336296.1	hypothetical protein	-
BE949_RS02895	232142..232363	-	222	WP_000560223.1	killing protein KilR	-
BE949_RS02900	232785..232937	-	153	WP_000379561.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	qnrS1	-	226137..280895	54758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T71147 WP_001317028.1 NZ_CP018976:c228064-227870 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T71147 NZ_CP018976:c228064-227870 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT71147 NZ_CP018976:227693-227871 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin