Detailed information of TA system TA071143

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30173..30437
• Replicon: plasmid pEC545_1
• Accession: NZ_CP018975
• Organism: Escherichia coli strain Ecol_545

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: BE949_RS01155
• Protein ID: WP_001387489.1
• Coordinates: 30173..30325 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	30377..30437 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE949_RS01130	25439..27607	+	2169	WP_015508354.1	DotA/TraY family protein	-
BE949_RS01135	27678..28340	+	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
BE949_RS01140	28412..28621	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
BE949_RS27745	29013..29189	+	177	WP_001054900.1	hypothetical protein	-
BE949_RS01145	29254..29550	-	297	WP_011264046.1	hypothetical protein	-
BE949_RS01150	29850..30101	+	252	WP_001291964.1	hypothetical protein	-
BE949_RS01155	30173..30325	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	30377..30437	+	61	NuclAT_0	-	Antitoxin
-	30377..30437	+	61	NuclAT_0	-	Antitoxin
-	30377..30437	+	61	NuclAT_0	-	Antitoxin
-	30377..30437	+	61	NuclAT_0	-	Antitoxin
BE949_RS01160	30640..31731	+	1092	WP_000426061.1	hypothetical protein	-
-	31801..31858	+	58	NuclAT_1	-	-
-	31801..31858	+	58	NuclAT_1	-	-
-	31801..31858	+	58	NuclAT_1	-	-
-	31801..31858	+	58	NuclAT_1	-	-
BE949_RS01165	32038..33246	+	1209	WP_001703845.1	IncI1-type conjugal transfer protein TrbA	-
BE949_RS01170	33265..34335	+	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-15	-	1..95926	95926

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T71143 WP_001387489.1 NZ_CP018975:c30325-30173 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T71143 NZ_CP018975:c30325-30173 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT71143 NZ_CP018975:30377-30437 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin