Detailed information of TA system TA071128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1916188..1916790
• Replicon: chromosome
• Accession: NZ_CP018970
• Organism: Escherichia coli strain Ecol_542

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: BE966_RS11585
• Protein ID: WP_000897302.1
• Coordinates: 1916479..1916790 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE966_RS11580
• Protein ID: WP_000356397.1
• Coordinates: 1916188..1916478 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE966_RS11540	1911495..1912424	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
BE966_RS11545	1912606..1912848	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
BE966_RS11550	1913138..1913986	+	849	WP_001038650.1	hypothetical protein	-
BE966_RS11555	1914302..1914751	+	450	WP_032140890.1	hypothetical protein	-
BE966_RS11560	1914936..1915154	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
BE966_RS11570	1915551..1915829	-	279	WP_001296612.1	hypothetical protein	-
BE966_RS11580	1916188..1916478	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BE966_RS11585	1916479..1916790	-	312	WP_000897302.1	hypothetical protein	Toxin
BE966_RS11590	1917019..1917927	+	909	WP_001385591.1	alpha/beta hydrolase	-
BE966_RS11595	1917991..1918932	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE966_RS11600	1918977..1919414	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BE966_RS11605	1919411..1920283	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BE966_RS11610	1920277..1920876	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1915891..1960936	45045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T71128 WP_000897302.1 NZ_CP018970:c1916790-1916479 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71128 NZ_CP018970:c1916790-1916479 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71128 WP_000356397.1 NZ_CP018970:c1916478-1916188 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71128 NZ_CP018970:c1916478-1916188 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416