Detailed information of TA system TA071106

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20521..20763
• Replicon: plasmid pEC542_1
• Accession: NZ_CP018969
• Organism: Escherichia coli strain Ecol_542

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE966_RS00505
• Protein ID: WP_001312861.1
• Coordinates: 20521..20679 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20723..20763 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE966_RS00455	15633..15860	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
BE966_RS00460	15948..16625	-	678	WP_001348626.1	PAS domain-containing protein	-
BE966_RS00465	16759..17142	-	384	WP_001151566.1	relaxosome protein TraM	-
BE966_RS00470	17473..18075	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BE966_RS00475	18372..19193	-	822	WP_001234469.1	DUF945 domain-containing protein	-
BE966_RS00480	19312..19599	-	288	WP_000107535.1	hypothetical protein	-
BE966_RS29055	19624..19830	-	207	WP_000275859.1	hypothetical protein	-
BE966_RS29060	19743..20078	-	336	WP_013023876.1	hypothetical protein	-
BE966_RS00505	20521..20679	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	20723..20763	-	41	NuclAT_1	-	Antitoxin
-	20723..20763	-	41	NuclAT_1	-	Antitoxin
-	20723..20763	-	41	NuclAT_1	-	Antitoxin
-	20723..20763	-	41	NuclAT_1	-	Antitoxin
-	22207..22393	-	187	NuclAT_0	-	-
-	22207..22393	-	187	NuclAT_0	-	-
-	22207..22393	-	187	NuclAT_0	-	-
-	22207..22393	-	187	NuclAT_0	-	-
BE966_RS00520	22405..23124	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
BE966_RS00525	23121..23555	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
BE966_RS00530	23610..25568	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-27	-	1..82364	82364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71106 WP_001312861.1 NZ_CP018969:c20679-20521 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71106 NZ_CP018969:c20679-20521 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT71106 NZ_CP018969:c20763-20723 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin