Detailed information of TA system TA071079

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5043..5297
• Replicon: plasmid pEC517_1
• Accession: NZ_CP018964
• Organism: Escherichia coli strain Ecol_517

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BE959_RS00390
• Protein ID: WP_015387399.1
• Coordinates: 5091..5297 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	5043..5104 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE959_RS00340	695..907	+	213	WP_005012601.1	hypothetical protein	-
BE959_RS26275	1208..1297	-	90	Protein_2	IS1 family transposase	-
BE959_RS00355	1352..2029	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
BE959_RS00360	2029..2376	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
BE959_RS00365	2396..3967	+	1572	WP_023149734.1	IS66 family transposase	-
BE959_RS00370	4005..4621	-	617	Protein_6	IS1 family transposase	-
BE959_RS00380	4722..4904	+	183	WP_000968309.1	hypothetical protein	-
-	5043..5104	-	62	NuclAT_0	-	Antitoxin
-	5043..5104	-	62	NuclAT_0	-	Antitoxin
-	5043..5104	-	62	NuclAT_0	-	Antitoxin
-	5043..5104	-	62	NuclAT_0	-	Antitoxin
BE959_RS00390	5091..5297	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE959_RS00395	5581..5838	+	258	WP_000083833.1	replication regulatory protein RepA	-
BE959_RS00405	6074..6148	+	75	WP_032336874.1	RepA leader peptide Tap	-
BE959_RS00410	6141..6587	+	447	Protein_11	incFII family plasmid replication initiator RepA	-
BE959_RS00415	6533..7015	-	483	Protein_12	VENN motif pre-toxin domain-containing protein	-
BE959_RS00420	7908..9128	+	1221	WP_000410951.1	arginine deiminase	-
BE959_RS00425	9139..10050	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / sul1 / qacE / aadA5	senB / iucD / iutA	1..118495	118495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T71079 WP_015387399.1 NZ_CP018964:5091-5297 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T71079 NZ_CP018964:5091-5297 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT71079 NZ_CP018964:c5104-5043 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin