71068

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3766748..3767310 Replicon chromosome
Accession NZ_CP018957
Organism Escherichia coli strain Ecol_316

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag BE963_RS21955 Protein ID WP_000605676.1
Coordinates 3767032..3767310 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag BE963_RS21950 Protein ID WP_000781370.1
Coordinates 3766748..3767032 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS21935 3761930..3764977 + 3048 WP_077577597.1 formate dehydrogenase-N subunit alpha -
BE963_RS21940 3764990..3765874 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
BE963_RS21945 3765867..3766520 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
BE963_RS21950 3766748..3767032 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
BE963_RS21955 3767032..3767310 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE963_RS21960 3767496..3768506 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
BE963_RS21965 3768640..3770337 - 1698 WP_000433464.1 malate dehydrogenase -
BE963_RS21970 3770494..3770631 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
BE963_RS21975 3770733..3770948 - 216 WP_000495766.1 biofilm-dependent modulation protein -
BE963_RS21985 3771293..3771724 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T71068 WP_000605676.1 NZ_CP018957:c3767310-3767032 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T71068 NZ_CP018957:c3767310-3767032 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT71068 WP_000781370.1 NZ_CP018957:c3767032-3766748 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT71068 NZ_CP018957:c3767032-3766748 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References