Detailed information of TA system TA071063

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3622983..3623354
• Replicon: chromosome
• Accession: NZ_CP018957
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: BE963_RS21175
• Protein ID: WP_001317028.1
• Coordinates: 3623160..3623354 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3622983..3623161 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS21150	3618938..3620311	+	1374	WP_000123754.1	ATP-dependent RNA helicase DbpA	-
BE963_RS21155	3620440..3621375	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
BE963_RS21160	3621427..3622662	-	1236	WP_000040858.1	site-specific integrase	-
BE963_RS21165	3622664..3622879	-	216	WP_000079604.1	excisionase XisR	-
BE963_RS21170	3622958..3623167	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	3622983..3623161	+	179	NuclAT_0	-	Antitoxin
-	3622983..3623161	+	179	NuclAT_0	-	Antitoxin
-	3622983..3623161	+	179	NuclAT_0	-	Antitoxin
-	3622983..3623161	+	179	NuclAT_0	-	Antitoxin
BE963_RS21175	3623160..3623354	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
BE963_RS21180	3623411..3624220	-	810	WP_000166319.1	recombination protein RecT	-
BE963_RS21185	3624213..3626813	-	2601	WP_001586992.1	exodeoxyribonuclease VIII	-
BE963_RS21190	3626915..3627190	-	276	WP_000632297.1	protein RacC	-
BE963_RS21195	3627265..3627435	-	171	WP_001352098.1	conserved protein, Rac prophage	-
BE963_RS21200	3627435..3627656	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3621427..3668979	47552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T71063 WP_001317028.1 NZ_CP018957:c3623354-3623160 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T71063 NZ_CP018957:c3623354-3623160 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT71063 NZ_CP018957:3622983-3623161 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin