Detailed information of TA system TA071054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2407029..2407723
• Replicon: chromosome
• Accession: NZ_CP018957
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: V0YKG6
• Locus tag: BE963_RS14895
• Protein ID: WP_001263485.1
• Coordinates: 2407325..2407723 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BE963_RS14890
• Protein ID: WP_000554758.1
• Coordinates: 2407029..2407322 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS14870	2402345..2402842	+	498	WP_000006263.1	REP-associated tyrosine transposase RayT	-
BE963_RS14875	2403382..2405094	-	1713	Protein_2208	flagellar biosynthesis protein FlhA	-
BE963_RS14880	2405066..2405851	+	786	WP_000207554.1	putative lateral flagellar export/assembly protein LafU	-
BE963_RS14885	2405922..2406977	+	1056	WP_001226177.1	DNA polymerase IV	-
BE963_RS14890	2407029..2407322	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BE963_RS14895	2407325..2407723	+	399	WP_001263485.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BE963_RS14900	2407733..2408185	+	453	WP_001059899.1	GNAT family N-acetyltransferase	-
BE963_RS14905	2408431..2408637	+	207	Protein_2214	RtcB family protein	-
BE963_RS14910	2408633..2408985	+	353	Protein_2215	peptide chain release factor H	-
BE963_RS14915	2409042..2410499	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
BE963_RS14920	2410760..2411218	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BE963_RS14925	2411310..2412554	+	1245	WP_001586695.1	esterase FrsA	-
-	2411814..2411894	+	81	NuclAT_12	-	-
-	2411814..2411894	+	81	NuclAT_12	-	-
-	2411814..2411894	+	81	NuclAT_12	-	-
-	2411814..2411894	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15418.84 Da
• Isoelectric Point: 7.3840

>T71054 WP_001263485.1 NZ_CP018957:2407325-2407723 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T71054 NZ_CP018957:2407325-2407723 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT71054 WP_000554758.1 NZ_CP018957:2407029-2407322 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT71054 NZ_CP018957:2407029-2407322 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: V0YKG6

Antitoxin

• AlphaFold DB: S1QAE3