Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 34852..35509 Replicon plasmid pEC316_KPC
Accession NZ_CP018956
Organism Escherichia coli strain Ecol_316

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag BE963_RS01580 Protein ID WP_000270043.1
Coordinates 34852..35202 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BE963_RS01585 Protein ID WP_000124640.1
Coordinates 35207..35509 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS01540 31326..31823 - 498 WP_000062185.1 hypothetical protein -
BE963_RS01545 31826..32314 - 489 WP_001273096.1 DUF1643 domain-containing protein -
BE963_RS01550 32411..32746 + 336 WP_000683476.1 hypothetical protein -
BE963_RS01555 32761..33231 - 471 WP_001281821.1 hypothetical protein -
BE963_RS01560 33224..33595 - 372 WP_000516916.1 hypothetical protein -
BE963_RS01565 33606..33800 - 195 WP_000343597.1 hypothetical protein -
BE963_RS01575 34141..34689 - 549 WP_001061574.1 transcriptional regulator -
BE963_RS01580 34852..35202 + 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE963_RS01585 35207..35509 + 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
BE963_RS01590 35536..35829 - 294 WP_001239997.1 chromosome segregation protein ParM -
BE963_RS01595 35917..36189 - 273 WP_001043047.1 HU family DNA-binding protein -
BE963_RS01600 36247..36792 - 546 WP_032413569.1 thermonuclease family protein -
BE963_RS01610 36916..37896 - 981 WP_012569499.1 IS5-like element ISKpn26 family transposase -
BE963_RS01615 37991..38179 - 189 WP_001270411.1 hypothetical protein -
BE963_RS01620 38205..39062 - 858 WP_001167032.1 hypothetical protein -
BE963_RS01625 39049..39279 - 231 WP_000972663.1 hypothetical protein -
BE963_RS01630 39279..39797 - 519 WP_000210758.1 nitrite reductase -
BE963_RS01635 39794..40240 - 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1D / ant(2'')-Ia / aac(6')-33 / qacE / sul1 / blaKPC-2 / sul2 / floR - 1..216226 216226
- flank IS/Tn - - 36916..37896 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-106)

Antitoxin

(33-79)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T71042 WP_000270043.1 NZ_CP018956:34852-35202 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T71042 NZ_CP018956:34852-35202 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT71042 WP_000124640.1 NZ_CP018956:35207-35509 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT71042 NZ_CP018956:35207-35509 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9


Antitoxin

Source ID Structure

References