Detailed information of TA system TA071023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2776557..2777159
• Replicon: chromosome
• Accession: NZ_CP018953
• Organism: Escherichia coli strain Ecol_276

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: BE964_RS15810
• Protein ID: WP_000897302.1
• Coordinates: 2776557..2776868 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BE964_RS15815
• Protein ID: WP_000356397.1
• Coordinates: 2776869..2777159 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE964_RS15785	2772471..2773070	+	600	WP_001296610.1	glucose-1-phosphatase	-
BE964_RS15790	2773064..2773936	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BE964_RS15795	2773933..2774370	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BE964_RS15800	2774415..2775356	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BE964_RS15805	2775420..2776328	-	909	WP_001385591.1	alpha/beta hydrolase	-
BE964_RS15810	2776557..2776868	+	312	WP_000897302.1	hypothetical protein	Toxin
BE964_RS15815	2776869..2777159	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BE964_RS15825	2777518..2777796	+	279	WP_001296612.1	hypothetical protein	-
BE964_RS15835	2778193..2778411	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
BE964_RS15840	2778596..2779045	-	450	WP_032140890.1	hypothetical protein	-
BE964_RS15845	2779361..2780209	-	849	WP_001038650.1	hypothetical protein	-
BE964_RS15850	2780499..2780741	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
BE964_RS15855	2780923..2781852	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T71023 WP_000897302.1 NZ_CP018953:2776557-2776868 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T71023 NZ_CP018953:2776557-2776868 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT71023 WP_000356397.1 NZ_CP018953:2776869-2777159 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT71023 NZ_CP018953:2776869-2777159 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416