71021

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1967331..1968130 Replicon chromosome
Accession NZ_CP018953
Organism Escherichia coli strain Ecol_276

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag BE964_RS11685 Protein ID WP_000347251.1
Coordinates 1967666..1968130 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag BE964_RS11680 Protein ID WP_001296435.1
Coordinates 1967331..1967666 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE964_RS11665 1963116..1963886 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
BE964_RS11670 1963902..1965236 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
BE964_RS11675 1965611..1967182 + 1572 WP_001273763.1 galactarate dehydratase -
BE964_RS11680 1967331..1967666 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
BE964_RS11685 1967666..1968130 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
BE964_RS11690 1968185..1968994 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
BE964_RS11700 1969243..1970523 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
BE964_RS11705 1970546..1971019 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
BE964_RS11710 1971030..1971809 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
BE964_RS11715 1971799..1972677 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
BE964_RS11720 1972695..1973129 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T71021 WP_000347251.1 NZ_CP018953:1967666-1968130 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T71021 NZ_CP018953:1967666-1968130 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT71021 WP_001296435.1 NZ_CP018953:1967331-1967666 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT71021 NZ_CP018953:1967331-1967666 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CJ20


Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References