Detailed information of TA system TA071004

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 116000..116239
• Replicon: plasmid pEC276_1
• Accession: NZ_CP018952
• Organism: Escherichia coli strain Ecol_276

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: BE964_RS01465
• Protein ID: WP_023144756.1
• Coordinates: 116105..116239 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	116000..116060 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE964_RS01430	111791..112351	+	561	WP_000139341.1	fertility inhibition protein FinO	-
BE964_RS01435	112482..112694	+	213	WP_013023861.1	hypothetical protein	-
BE964_RS01445	113253..113678	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
BE964_RS01450	113675..114025	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
BE964_RS01455	114056..115669	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
BE964_RS27360	115747..116033	+	287	Protein_130	DUF2726 domain-containing protein	-
-	116000..116060	-	61	NuclAT_2	-	Antitoxin
-	116000..116060	-	61	NuclAT_2	-	Antitoxin
-	116000..116060	-	61	NuclAT_2	-	Antitoxin
-	116000..116060	-	61	NuclAT_2	-	Antitoxin
BE964_RS01465	116105..116239	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE964_RS01470	116536..116790	+	255	WP_000083850.1	replication regulatory protein RepA	-
BE964_RS27505	116896..117030	+	135	Protein_133	protein CopA/IncA	-
BE964_RS01480	117027..117101	+	75	WP_031943482.1	RepA leader peptide Tap	-
BE964_RS01485	117094..117951	+	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
BE964_RS01495	118891..119544	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BE964_RS27000	119637..119894	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BE964_RS01500	119827..120228	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..132345	132345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T71004 WP_023144756.1 NZ_CP018952:116105-116239 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T71004 NZ_CP018952:116105-116239 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT71004 NZ_CP018952:c116060-116000 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin