Detailed information of TA system TA071000

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74160..74402
• Replicon: plasmid pEC276_1
• Accession: NZ_CP018952
• Organism: Escherichia coli strain Ecol_276

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BE964_RS01210
• Protein ID: WP_001312861.1
• Coordinates: 74244..74402 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	74160..74200 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE964_RS01185	69355..71313	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
BE964_RS01190	71368..71802	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
BE964_RS01195	71799..72518	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	72530..72716	+	187	NuclAT_0	-	-
-	72530..72716	+	187	NuclAT_0	-	-
-	72530..72716	+	187	NuclAT_0	-	-
-	72530..72716	+	187	NuclAT_0	-	-
BE964_RS01205	72764..74133	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	74160..74200	+	41	NuclAT_1	-	Antitoxin
-	74160..74200	+	41	NuclAT_1	-	Antitoxin
-	74160..74200	+	41	NuclAT_1	-	Antitoxin
-	74160..74200	+	41	NuclAT_1	-	Antitoxin
BE964_RS01210	74244..74402	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE964_RS27500	74845..75180	+	336	WP_013023876.1	hypothetical protein	-
BE964_RS27355	75093..75299	+	207	WP_000275859.1	hypothetical protein	-
BE964_RS01235	75324..75611	+	288	WP_000107535.1	hypothetical protein	-
BE964_RS01240	75730..76551	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BE964_RS01245	76848..77450	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
BE964_RS01250	77781..78164	+	384	WP_001151566.1	relaxosome protein TraM	-
BE964_RS01255	78298..78975	+	678	WP_001348626.1	PAS domain-containing protein	-
BE964_RS01260	79063..79290	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..132345	132345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T71000 WP_001312861.1 NZ_CP018952:74244-74402 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T71000 NZ_CP018952:74244-74402 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT71000 NZ_CP018952:74160-74200 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin