Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2069949..2070451 Replicon chromosome
Accession NZ_CP018948
Organism Escherichia coli strain Ecol_224

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BE957_RS12870 Protein ID WP_000767829.1
Coordinates 2070197..2070451 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BE957_RS12865 Protein ID WP_001259255.1
Coordinates 2069949..2070200 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS12835 2065127..2066194 - 1068 WP_032248774.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BE957_RS12840 2066194..2067264 - 1071 WP_078207164.1 histidinol-phosphate transaminase -
BE957_RS12845 2067261..2068565 - 1305 WP_001345878.1 histidinol dehydrogenase -
BE957_RS12850 2068571..2069470 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BE957_RS12860 2069616..2069666 - 51 WP_001364200.1 his operon leader peptide -
BE957_RS12865 2069949..2070200 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BE957_RS12870 2070197..2070451 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BE957_RS12875 2070534..2071358 + 825 WP_000754737.1 SDR family oxidoreductase -
BE957_RS12880 2071404..2072333 + 930 WP_000803351.1 LysR family transcriptional regulator -
BE957_RS27965 2072548..2072610 + 63 WP_010723108.1 membrane protein YoeI -
BE957_RS12885 2072600..2073958 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BE957_RS12890 2074137..2075195 + 1059 WP_000492324.1 transport protein YeeE -
BE957_RS12895 2075209..2075436 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T70978 WP_000767829.1 NZ_CP018948:2070197-2070451 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T70978 NZ_CP018948:2070197-2070451 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT70978 WP_001259255.1 NZ_CP018948:2069949-2070200 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT70978 NZ_CP018948:2069949-2070200 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References