Detailed information of TA system TA070965

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29350..29771
• Replicon: plasmid pEC224_1
• Accession: NZ_CP018947
• Organism: Escherichia coli strain Ecol_224

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BE957_RS01425
• Protein ID: WP_001302184.1
• Coordinates: 29613..29771 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29350..29548 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE957_RS01395	25046..26440	+	1395	WP_001037799.1	carbohydrate porin	-
BE957_RS27800	26686..26790	-	105	Protein_27	thiosulfate reductase cytochrome B subunit	-
BE957_RS01400	26847..27540	+	694	Protein_28	IS1 family transposase	-
BE957_RS01405	27556..28179	+	624	WP_000912556.1	potassium channel family protein	-
BE957_RS01410	28264..28515	+	252	Protein_30	iron-containing alcohol dehydrogenase	-
BE957_RS01415	28804..29121	-	318	Protein_31	IS110 family transposase	-
BE957_RS01420	29276..29381	+	106	Protein_32	plasmid SOS inhibition protein A	-
-	29350..29548	+	199	NuclAT_0	-	Antitoxin
-	29350..29548	+	199	NuclAT_0	-	Antitoxin
-	29350..29548	+	199	NuclAT_0	-	Antitoxin
-	29350..29548	+	199	NuclAT_0	-	Antitoxin
BE957_RS01425	29613..29771	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
BE957_RS28245	30439..30645	+	207	WP_000275854.1	hypothetical protein	-
BE957_RS01440	30670..30957	+	288	WP_000107242.1	hypothetical protein	-
BE957_RS01445	31076..31897	+	822	WP_001234465.1	DUF945 domain-containing protein	-
BE957_RS01450	32193..32765	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
BE957_RS01455	33135..33518	+	384	WP_001063020.1	relaxosome protein TraM	-
BE957_RS01460	33709..34356	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
BE957_RS01465	34492..34707	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / tet(B)	-	1..143972	143972
	-	flank	IS/Tn	-	-	27163..27540	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T70965 WP_001302184.1 NZ_CP018947:29613-29771 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T70965 NZ_CP018947:29613-29771 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT70965 NZ_CP018947:29350-29548 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin