Detailed information of TA system TA070915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3913075..3913805
• Replicon: chromosome
• Accession: NZ_CP018928
• Organism: Serratia marcescens strain UMH10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: BVG92_RS18640
• Protein ID: WP_004937033.1
• Coordinates: 3913075..3913386 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BVG92_RS18645
• Protein ID: WP_049208318.1
• Coordinates: 3913383..3913805 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVG92_RS18610	3908388..3909113	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
BVG92_RS18615	3909166..3910599	+	1434	WP_043141961.1	RNA polymerase factor sigma-54	-
BVG92_RS18620	3910623..3910910	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
BVG92_RS18625	3911066..3911527	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
BVG92_RS18630	3911657..3912511	+	855	WP_015379177.1	RNase adapter RapZ	-
BVG92_RS18635	3912508..3912780	+	273	WP_043141958.1	PTS phosphocarrier protein NPr	-
BVG92_RS18640	3913075..3913386	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
BVG92_RS18645	3913383..3913805	+	423	WP_049208318.1	helix-turn-helix domain-containing protein	Antitoxin
BVG92_RS18650	3913810..3915726	-	1917	WP_049208316.1	BglG family transcription antiterminator	-
BVG92_RS18655	3915778..3916992	-	1215	WP_089181374.1	lactonase family protein	-
BVG92_RS18660	3917204..3917944	-	741	WP_089181375.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T70915 WP_004937033.1 NZ_CP018928:3913075-3913386 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T70915 NZ_CP018928:3913075-3913386 [Serratia marcescens]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTACGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCGTCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15765.08 Da
• Isoelectric Point: 5.1994

>AT70915 WP_049208318.1 NZ_CP018928:3913383-3913805 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSHAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT70915 NZ_CP018928:3913383-3913805 [Serratia marcescens]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTACCCTTATTGCAGGGCAGCCACGCTCAGCAAGA
CTACCGAGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACACCATCCGCTGATTGACATGTTGGCAAAAA
AAATCGCCGAATATGAAGACAACAGTGCCGAATTTACTGCATTCAACCAGCGTATCGCCCAACTCCCGCAGGGGGTGGCC
GCGCTTCGCGTATTGATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B4GR63