Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2113810..2114306 Replicon chromosome
Accession NZ_CP018840
Organism Escherichia coli strain KSC64

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag BUQ71_RS10765 Protein ID WP_032163111.1
Coordinates 2114154..2114306 (+) Length 51 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BUQ71_RS10760 Protein ID WP_000398619.1
Coordinates 2113810..2114106 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BUQ71_RS10735 2110541..2111260 - 720 WP_000611288.1 two-component system response regulator DcuR -
BUQ71_RS10740 2111257..2112888 - 1632 WP_001216486.1 sensor histidine kinase -
BUQ71_RS10745 2113069..2113299 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
BUQ71_RS10750 2113311..2113583 + 273 WP_000405647.1 N-acetyltransferase -
BUQ71_RS10760 2113810..2114106 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BUQ71_RS10765 2114154..2114306 + 153 WP_032163111.1 GhoT/OrtT family toxin Toxin
BUQ71_RS10770 2114425..2115942 - 1518 WP_001295074.1 lysine--tRNA ligase -
BUQ71_RS10775 2116179..2117636 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2113810..2123159 9349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(3-50)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5764.99 Da        Isoelectric Point: 8.7996

>T70670 WP_032163111.1 NZ_CP018840:2114154-2114306 [Escherichia coli]
INFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 153 bp

>T70670 NZ_CP018840:2114154-2114306 [Escherichia coli]
ATTAATTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTT
ACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT70670 WP_000398619.1 NZ_CP018840:2113810-2114106 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT70670 NZ_CP018840:2113810-2114106 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References