Detailed information of TA system TA070658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 423694..424324
• Replicon: chromosome
• Accession: NZ_CP018839
• Organism: Thauera chlorobenzoica strain 3CB1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1H5ZCP6
• Locus tag: Tchl_RS02050
• Protein ID: WP_075146923.1
• Coordinates: 423998..424324 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1H5Z9B9
• Locus tag: Tchl_RS02045
• Protein ID: WP_075146922.1
• Coordinates: 423694..424005 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tchl_RS02010	418790..419419	-	630	WP_146060811.1	class I SAM-dependent methyltransferase	-
Tchl_RS02015	419438..420760	-	1323	WP_075146916.1	UDP-glucose/GDP-mannose dehydrogenase family protein	-
Tchl_RS02020	420967..421191	+	225	WP_146060812.1	hypothetical protein	-
Tchl_RS02030	421588..422625	+	1038	WP_075146919.1	Rpn family recombination-promoting nuclease/putative transposase	-
Tchl_RS02035	422754..423035	-	282	WP_075146920.1	helix-turn-helix domain-containing protein	-
Tchl_RS02040	423032..423370	-	339	WP_075146921.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Tchl_RS02045	423694..424005	-	312	WP_075146922.1	helix-turn-helix domain-containing protein	Antitoxin
Tchl_RS02050	423998..424324	-	327	WP_075146923.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Tchl_RS02055	424485..425570	+	1086	WP_075146924.1	IS5 family transposase	-
Tchl_RS17665	425800..425913	+	114	Protein_407	hypothetical protein	-
Tchl_RS17550	425983..426231	+	249	WP_160113134.1	ribbon-helix-helix protein, CopG family	-
Tchl_RS02070	426228..426647	+	420	WP_075146925.1	PIN domain-containing protein	-
Tchl_RS02075	426807..428492	+	1686	WP_075146926.1	PilZ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	421588..424324	2736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-95)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12604.63 Da
• Isoelectric Point: 9.0634

>T70658 WP_075146923.1 NZ_CP018839:c424324-423998 [Thauera chlorobenzoica]
VNWTVSYFNERVQREITEWPVGIYADYLRLLLLMEEHGADLRLPHSRAMGDGLFELRCKGAEGIGRAFYCTMVGREIVIL
HSFIKKTQETPDRELKTARKRLKEVKHA

Nucleotide

• Length: 327 bp

>T70658 NZ_CP018839:c424324-423998 [Thauera chlorobenzoica]
GTGAACTGGACTGTCAGTTACTTCAACGAGCGCGTGCAACGCGAGATCACCGAGTGGCCGGTTGGCATCTACGCCGACTA
CCTTCGCCTGCTCTTACTGATGGAAGAGCACGGTGCCGACCTACGCCTGCCGCACTCACGTGCCATGGGCGACGGACTCT
TCGAGTTACGTTGCAAGGGCGCGGAAGGAATTGGCCGTGCGTTCTACTGCACGATGGTCGGACGCGAGATTGTGATCCTG
CACAGCTTTATCAAGAAGACGCAGGAAACGCCCGATCGGGAACTGAAGACAGCCCGCAAGCGACTGAAGGAGGTCAAACA
TGCATAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11538.27 Da
• Isoelectric Point: 10.2589

>AT70658 WP_075146922.1 NZ_CP018839:c424005-423694 [Thauera chlorobenzoica]
MHSEQFKPVRLDTKAELARAMQRPGFKAAWDGLEEEYAALNELLQARKQAGLTQEEVAARMGTTKSAVSRLEASIRSEHH
SPSFATLRKYAHACGKRLVIKMV

Nucleotide

• Length: 312 bp

>AT70658 NZ_CP018839:c424005-423694 [Thauera chlorobenzoica]
ATGCATAGTGAGCAATTCAAGCCGGTTCGCCTGGACACCAAGGCGGAACTTGCCCGTGCCATGCAACGGCCAGGATTCAA
GGCCGCGTGGGACGGCCTGGAAGAAGAGTACGCTGCACTCAACGAACTGCTGCAGGCCCGCAAGCAGGCGGGCCTGACCC
AAGAGGAAGTCGCAGCCCGCATGGGAACGACCAAGAGTGCTGTGTCGCGCCTGGAAGCCTCGATCCGCAGCGAACACCAT
TCGCCCTCGTTCGCCACGCTGCGCAAGTACGCACATGCCTGCGGCAAGCGCCTGGTCATCAAGATGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A1H5ZCP6

Antitoxin

• AlphaFold DB: A0A1H5Z9B9