Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 177458..177993 Replicon plasmid p1_ISMMS_VRE9
Accession NZ_CP018832
Organism Enterococcus faecium strain ISMMS_VRE_9

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag AL023_RS16175 Protein ID WP_002305108.1
Coordinates 177458..177730 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag AL023_RS16180 Protein ID WP_002303154.1
Coordinates 177730..177993 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL023_RS16145 172464..173651 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
AL023_RS16150 173839..174786 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
AL023_RS16155 175083..175631 - 549 WP_002292681.1 hypothetical protein -
AL023_RS16160 175632..176489 - 858 WP_002292680.1 AAA family ATPase -
AL023_RS16165 176775..177062 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
AL023_RS16170 177052..177381 - 330 WP_002292678.1 AbrB family transcriptional regulator -
AL023_RS16175 177458..177730 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
AL023_RS16180 177730..177993 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL023_RS16185 178099..178515 - 417 WP_002303153.1 hypothetical protein -
AL023_RS16190 178521..179084 - 564 Protein_195 IS6 family transposase -
AL023_RS16200 179164..180326 + 1163 WP_102642289.1 IS3 family transposase -
AL023_RS16205 180798..181697 - 900 WP_002303235.1 aldose 1-epimerase family protein -
AL023_RS16210 181718..181990 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..259837 259837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T70649 WP_002305108.1 NZ_CP018832:c177730-177458 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T70649 NZ_CP018832:c177730-177458 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT70649 WP_002303154.1 NZ_CP018832:c177993-177730 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT70649 NZ_CP018832:c177993-177730 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References