Detailed information of TA system TA070631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 273208..273721
• Replicon: chromosome
• Accession: NZ_CP018822
• Organism: Streptococcus gallolyticus subsp. gallolyticus DSM 16831

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A1S5W9N2
• Locus tag: BTR42_RS01575
• Protein ID: WP_049533167.1
• Coordinates: 273208..273462 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: BTR42_RS01580
• Protein ID: WP_077496080.1
• Coordinates: 273455..273721 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTR42_RS01540	268860..269276	-	417	WP_077496066.1	conjugal transfer protein	-
BTR42_RS01545	269278..269505	-	228	WP_077496068.1	hypothetical protein	-
BTR42_RS01550	269522..270523	-	1002	WP_077496070.1	conjugal transfer protein	-
BTR42_RS01555	270533..271087	-	555	WP_077496072.1	conjugal transfer protein	-
BTR42_RS01560	271105..271542	-	438	WP_077496074.1	hypothetical protein	-
BTR42_RS01565	271557..271943	-	387	WP_077496076.1	hypothetical protein	-
BTR42_RS01570	271921..273153	-	1233	WP_077496078.1	replication initiation factor domain-containing protein	-
BTR42_RS01575	273208..273462	-	255	WP_049533167.1	Txe/YoeB family addiction module toxin	Toxin
BTR42_RS01580	273455..273721	-	267	WP_077496080.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BTR42_RS01585	273984..275642	-	1659	WP_077496082.1	cell division protein FtsK	-
BTR42_RS01590	275649..276059	-	411	WP_003041912.1	DUF961 family protein	-
BTR42_RS01595	276082..276396	-	315	WP_000406626.1	hypothetical protein	-
BTR42_RS01600	276511..276738	-	228	WP_077496084.1	TetR family transcriptional regulator	-
BTR42_RS01605	276735..276971	-	237	WP_077496086.1	hypothetical protein	-
BTR42_RS01610	277100..277378	-	279	WP_077496088.1	type II toxin-antitoxin system YafQ family toxin	-
BTR42_RS01615	277379..277651	-	273	WP_077496090.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
BTR42_RS01620	278325..278687	+	363	WP_049533221.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	220747..290158	69411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10492.93 Da
• Isoelectric Point: 8.3800

>T70631 WP_049533167.1 NZ_CP018822:c273462-273208 [Streptococcus gallolyticus subsp. gallolyticus DSM 16831]
MFNFTEQAWKDYVSWQQEDKKILKRINRLIEDIKRNPFEGIGKPEPLKYRYSGAWSRRITDEHRLVYMVEEDEIYFLSFR
DHYK

Nucleotide

• Length: 255 bp

>T70631 NZ_CP018822:c273462-273208 [Streptococcus gallolyticus subsp. gallolyticus DSM 16831]
ATGTTTAACTTTACAGAACAAGCTTGGAAAGATTATGTTTCTTGGCAACAAGAAGATAAAAAGATACTTAAACGTATCAA
TCGTTTAATTGAGGATATCAAACGAAATCCTTTTGAAGGGATTGGAAAGCCTGAACCCTTAAAATACCGTTATTCTGGTG
CTTGGTCTCGTAGAATTACAGATGAACACCGATTAGTCTATATGGTGGAAGAAGATGAAATTTATTTCTTATCCTTTCGA
GACCATTATAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10404.98 Da
• Isoelectric Point: 5.1486

>AT70631 WP_077496080.1 NZ_CP018822:c273721-273455 [Streptococcus gallolyticus subsp. gallolyticus DSM 16831]
MEAVVYSNFRNNLKNYMKKVNDEYEPLMVVNKNPDENIVVLSKENWDSIQETIRLMNNDYLSDKVLSGMEQVKQKKVIQR
PLLEVKDV

Nucleotide

• Length: 267 bp

>AT70631 NZ_CP018822:c273721-273455 [Streptococcus gallolyticus subsp. gallolyticus DSM 16831]
ATGGAAGCTGTCGTCTATTCAAATTTTAGAAACAATTTAAAGAATTACATGAAAAAAGTCAACGATGAATATGAACCTCT
AATGGTTGTCAATAAAAATCCTGATGAAAATATTGTGGTCTTATCTAAGGAAAACTGGGATAGTATTCAAGAAACTATCC
GCCTAATGAATAACGATTATCTTTCAGACAAGGTTCTATCAGGAATGGAACAAGTCAAGCAAAAGAAAGTCATTCAACGC
CCATTATTAGAGGTGAAAGATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A1S5W9N2