Detailed information of TA system TA070563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3781110..3781804
• Replicon: chromosome
• Accession: NZ_CP018801
• Organism: Escherichia coli strain tolC-

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: BTD92_RS20380
• Protein ID: WP_001263489.1
• Coordinates: 3781110..3781508 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BTD92_RS20385
• Protein ID: WP_000554758.1
• Coordinates: 3781511..3781804 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3776698..3776778	-	81	NuclAT_13	-	-
-	3776698..3776778	-	81	NuclAT_13	-	-
-	3776698..3776778	-	81	NuclAT_13	-	-
-	3776698..3776778	-	81	NuclAT_13	-	-
BTD92_RS20350	3777374..3777832	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BTD92_RS20355	3778093..3779550	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
BTD92_RS20360	3779607..3780128	-	522	Protein_3629	peptide chain release factor H	-
BTD92_RS20365	3780124..3780330	-	207	Protein_3630	RtcB family protein	-
BTD92_RS20375	3780648..3781100	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
BTD92_RS20380	3781110..3781508	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BTD92_RS20385	3781511..3781804	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BTD92_RS20390	3781856..3782911	-	1056	WP_001226164.1	DNA polymerase IV	-
BTD92_RS20395	3782982..3783767	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
BTD92_RS20400	3783739..3785451	+	1713	Protein_3636	flagellar biosynthesis protein FlhA	-
BTD92_RS20410	3785675..3786172	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T70563 WP_001263489.1 NZ_CP018801:c3781508-3781110 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T70563 NZ_CP018801:c3781508-3781110 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT70563 WP_000554758.1 NZ_CP018801:c3781804-3781511 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT70563 NZ_CP018801:c3781804-3781511 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3