Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3781110..3781804 Replicon chromosome
Accession NZ_CP018801
Organism Escherichia coli strain tolC-

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag BTD92_RS20380 Protein ID WP_001263489.1
Coordinates 3781110..3781508 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag BTD92_RS20385 Protein ID WP_000554758.1
Coordinates 3781511..3781804 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3776698..3776778 - 81 NuclAT_13 - -
- 3776698..3776778 - 81 NuclAT_13 - -
- 3776698..3776778 - 81 NuclAT_13 - -
- 3776698..3776778 - 81 NuclAT_13 - -
BTD92_RS20350 3777374..3777832 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
BTD92_RS20355 3778093..3779550 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
BTD92_RS20360 3779607..3780128 - 522 Protein_3629 peptide chain release factor H -
BTD92_RS20365 3780124..3780330 - 207 Protein_3630 RtcB family protein -
BTD92_RS20375 3780648..3781100 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
BTD92_RS20380 3781110..3781508 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BTD92_RS20385 3781511..3781804 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BTD92_RS20390 3781856..3782911 - 1056 WP_001226164.1 DNA polymerase IV -
BTD92_RS20395 3782982..3783767 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
BTD92_RS20400 3783739..3785451 + 1713 Protein_3636 flagellar biosynthesis protein FlhA -
BTD92_RS20410 3785675..3786172 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T70563 WP_001263489.1 NZ_CP018801:c3781508-3781110 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T70563 NZ_CP018801:c3781508-3781110 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT70563 WP_000554758.1 NZ_CP018801:c3781804-3781511 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT70563 NZ_CP018801:c3781804-3781511 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References