Detailed information of TA system TA070557

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 3404729..3405150
• Replicon: chromosome
• Accession: NZ_CP018801
• Organism: Escherichia coli strain tolC-

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BTD92_RS18235
• Protein ID: WP_001302184.1
• Coordinates: 3404729..3404887 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3404952..3405150 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTD92_RS18200	3400126..3400815	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
BTD92_RS18205	3401002..3401385	-	384	WP_001151527.1	relaxosome protein TraM	-
BTD92_RS18210	3401718..3402308	+	591	WP_170919186.1	transglycosylase SLT domain-containing protein	-
BTD92_RS18215	3402604..3403425	-	822	WP_001234417.1	DUF945 domain-containing protein	-
BTD92_RS18220	3403544..3403831	-	288	WP_000107542.1	hypothetical protein	-
BTD92_RS26810	3403856..3404062	-	207	WP_000547965.1	hypothetical protein	-
BTD92_RS26625	3404132..3404491	+	360	Protein_3267	hypothetical protein	-
BTD92_RS18235	3404729..3404887	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	3404952..3405150	-	199	NuclAT_1	-	Antitoxin
-	3404952..3405150	-	199	NuclAT_1	-	Antitoxin
-	3404952..3405150	-	199	NuclAT_1	-	Antitoxin
-	3404952..3405150	-	199	NuclAT_1	-	Antitoxin
BTD92_RS18245	3405162..3405881	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
BTD92_RS18250	3405878..3406312	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
BTD92_RS18255	3406367..3408325	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
BTD92_RS18260	3408391..3408624	-	234	WP_000005990.1	DUF905 family protein	-
BTD92_RS18265	3408681..3409220	-	540	WP_000290801.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	entA / entB / entE / entC / fepB / entS / fepD / fepG / fepC / entF / fes / fepA / entD	3254871..3444860	189989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T70557 WP_001302184.1 NZ_CP018801:c3404887-3404729 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T70557 NZ_CP018801:c3404887-3404729 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT70557 NZ_CP018801:c3405150-3404952 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin