Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1796547..1797049 Replicon chromosome
Accession NZ_CP018801
Organism Escherichia coli strain tolC-

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BTD92_RS09280 Protein ID WP_000767829.1
Coordinates 1796795..1797049 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BTD92_RS09275 Protein ID WP_001259255.1
Coordinates 1796547..1796798 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTD92_RS09245 1791725..1792792 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BTD92_RS09250 1792792..1793862 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
BTD92_RS09255 1793859..1795163 - 1305 WP_000009594.1 histidinol dehydrogenase -
BTD92_RS09260 1795169..1796068 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BTD92_RS09270 1796214..1796264 - 51 WP_001364200.1 his operon leader peptide -
BTD92_RS09275 1796547..1796798 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BTD92_RS09280 1796795..1797049 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BTD92_RS09285 1797132..1797956 + 825 WP_000754737.1 SDR family oxidoreductase -
BTD92_RS09290 1798002..1798931 + 930 WP_000803351.1 LysR family transcriptional regulator -
BTD92_RS26140 1799146..1799208 + 63 WP_010723108.1 membrane protein YoeI -
BTD92_RS09295 1799198..1800556 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BTD92_RS09300 1800735..1801793 + 1059 WP_000492339.1 transport protein YeeE -
BTD92_RS09305 1801807..1802034 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T70543 WP_000767829.1 NZ_CP018801:1796795-1797049 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T70543 NZ_CP018801:1796795-1797049 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT70543 WP_001259255.1 NZ_CP018801:1796547-1796798 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT70543 NZ_CP018801:1796547-1796798 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References